Deuterium metabolic imaging (DMI) is a novel 3D method to image metabolism of deuterium-labeled substrates in healthy or diseased human brain. DMI provides a powerful tool to reveal altered metabolism and provides completely novel information compared to standard MRI. Examples of aberrant metabolism in aggressive tumors demonstrate the unique metabolic dimension that DMI adds to the standard MRI arsenal. A range of biologically relevant, affordable deuterated substrates are available to probe multiple metabolic pathways. The robustness of DMI together with the high sensitivity, ease-of-use and affordability makes this novel technique highly relevant for 3D mapping of human brain metabolism.