Speaker
Description
Those of us engaged in active experimental research careers rarely have the opportunity to step back from the lab bench or the computer to reflect on our own scientific practice. Long before descriptive terms such as “multidisciplinary” or “integrative” were fashionable, biological NMR (BioNMR), from its very beginnings, was multidisciplinary in and by itself. Integration of complementary data has a long history in BioNMR and by now everyone in the scientific community is well aware that single types of methodologies are insufficient to adequately describe complex biological structures. I will describe the benefits of integrating solution NMR, MAS solid state NMR, crystallography, cryoEM and large-scale MD simulation, which permitted us to derive a realistic all-atom model for the entire HIV-1 capsid.