25-30 August 2019
Henry Ford Building
Europe/Berlin timezone

Synthesis of deuterated n-Dodecyl-β-D-Melibioside detergent for membrane protein solubilization

Not scheduled
4h
Harnack House and Henry Ford Building

Harnack House and Henry Ford Building

Board: 586
Poster-Late Late-Breaking Posters

Speaker

Ms Denitsa Radeva (FB Reagents Ltd)

Description

Lipids and detergents used for solubilization of membrane proteins can be deuterium-enriched to improve the quality of protein NMR spectra. Alkyl glycoside detergents have been instrumental in the X-ray structure determination of a number of GPCRs, starting with the first structures of the beta-adrenergic receptor solubilized in dodecyl maltoside (DDM). DDMB is a promising newer detergent that forms significantly smaller micelles that its isomer DDM. Here we describe the synthesis and purification of d25-dodecyl melibioside (DDMB) deuterated at the C12 tail.

Primary authors

Dr Vladimir Gelev (Sofia University) Ms Denitsa Radeva (FB Reagents Ltd)

Presentation Materials

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