25-30 August 2019
Henry Ford Building
Europe/Berlin timezone

Allosteric Activation of GDP-Bound Ras Isoforms by Bisphenol Derivative Plasticisers

Not scheduled
4h
Harnack House and Henry Ford Building

Harnack House and Henry Ford Building

Board: 433
Poster Posters

Speaker

Mr Oleksandr Shkura (Ruhr University of Bochum (Germany))

Description

The protein family of small GTPases controls cellular processes by acting as a binary switch between an active and an inactive state. The most prominent family members are H Ras, N Ras, and K Ras isoforms, which are highly related and frequently mutated in cancer. Bisphenols are widespread in modern life because of their industrial application as plasticisers. Bisphenol A (BPA) is the best known member and has gained significant scientific as well as public attention as an endocrine disrupting chemical, a fact that eventually led to its replacement. However, compounds used to replace BPA still contain the molecular scaffold of bisphenols. BPA, BPAF, BPB, BPE, BPF and an amine substituted BPAF derivate all interact with all GDP-bound Ras Isoforms through binding to a common site on these proteins. NMR-, SOScat-, and GDI- assay based data revealed a new Bisphenol-induced, allosterically activated GDP bound Ras conformation that define these plasticisers as Ras allosteric agonists.

Primary authors

Mrs Miriam Schöpel (Ruhr University of Bochum (Germany)) Mr Oleksandr Shkura (Ruhr University of Bochum (Germany)) Mrs Jana Seidel (Ruhr University of Bochum (Germany)) Mr Klaus Kock (Ruhr University of Bochum (Germany)) Mr Xueyin Zhong (Ruhr University of Bochum (Germany)) Mrs Stefanie Löffek (University Duisburg-Essen (Germany)) Mrs Iris Helfrich (University Duisburg-Essen (Germany)) Mr Hagen S. Bachmann (Witten/Herdecke University (Germany)) Mr Jürgen Scherkenbeck (University of Wuppertal (Germany)) Mr Raphael Stoll (Ruhr University of Bochum (Germany))

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