25-30 August 2019
Henry Ford Building
Europe/Berlin timezone

Complex Formation of the Tetracycline‐Binding Aptamer Investigated by Specific Cross‐Relaxation under DNP

28 Aug 2019, 13:35
Max Kade Auditorium (Henry Ford Building)

Max Kade Auditorium

Henry Ford Building

Prize lecture Hyperpolarization techniques Prize Lectures


Victoria Aladin (Goethe University Frankfurt)


Specific Cross-Relaxation Enhancement by Active Motions under DNP (SCREAM-DNP) is a method which relies on direct polarization transfer in solid-state DNP at typical DNP temperatures.[1] The mechanism is based on cross relaxation between 1H and 13C. It is generated by the internal dynamics of methyl groups resulting in negative enhancement and inverted 13C MAS NMR signal in a direct DNP experiment.[2] Furthermore, this effect can be suppressed by 1H saturation. Through mathematical subtraction, we can exclusively observe magnetization which was generated by cross relaxation. Therefore, the use of methyl groups as a specific promotor for polarization transfer opens new applications in DNP.
In this work, we show the application of SCREAM-DNP on a tetracycline-binding RNA aptamer. Here, CH3 groups were introduced into the biomolecular complex by non-covalent interaction between the aptamer and its highly specific ligand. Thereby, we use tetracycline which carries three CH3-groups as a source of cross-relaxation enhancement for complex formation studies. Moreover, we can influence the reorientation dynamics of methyl groups to a significant degree by changing the temperature, resulting in an increase of cross-relaxation efficiency. In conclusion, SCREAM-DNP is a promising method for different applications, especially in site-specific DNP-studies.
[1] Aladin et al., Angew. Chem. Int. Ed. 2019, 58, 4863.
[2] Daube et al., J. Am. Chem. Soc. 2016, 138, 16572.

Primary authors

Victoria Aladin (Goethe University Frankfurt) Dr Marc Vogel (TU Darmstadt) Dr Robert Binder (Goethe University Frankfurt) Prof. Irene Burghardt (Goethe University Frankfurt) Prof. Beatrix Suess (TU Darmstadt) Björn Corzilius (University of Rostock)

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